Every action in your lab should lead to efficiency and quality. That means automating processes as much as possible. To determine what steps can be repeated we break down how essays work. Based on those elements we design specific software and hardware. That way we know everything is aligned.
The Enzyme-Linked ImmunoSpot (ELISpot) assay is a widely-used method for monitoring cellular immune responses.
It is based on the principle of an ELISA, but works with cells. Rather than measuring the presence of antibodies or antigens in a suspension (as is done in ELISA).
The ELISpot (Enzyme Linked Immuno Spot) assay will measure the presence of antibody-secreting cells (or cytokine-secreting cells). In the end, the secreting cells are washed away but the antibodies or cytokine they have secreted can be isualised. They can be observed under a microscope as spots (hence the name), which can be counted.
Teaching and Reading software from MicroTechniX determines the number, size and intensities of the spots automatically.
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