Single Radial Diffusion

Single radial diffusion or (SRD) is an immuno-diffusion technique used in immunology to determine the quantity of an antigen.

Typically, wells are made in an agar gel that holds an antibody suspension. The antibody is directed against the antigen to be quantified. When antigen is added to the wells, complexes with the antibody will be formed. These complexes are soluble when the antigen is in excess, but as the antigen diffuses in the agar gel; and it's concentration decreases, insoluble precipitin complexes will form, which can be seen as circles.

By measuring the diameter of these circles and using a standard curve with known antigen concentrations, the quantity of the antigen can be calculated.

MicroTechniX can help you monitoring using the MacroLab II, Macroscan in combination with our software tool Icarus.


Every action in your lab should lead to efficiency and quality. That means automating processes as much as possible. To determine what steps can be repeated we break down how essays work. Based on those elements we design specific software and hardware. That way we know everything is aligned.

Wouter Christiaens
Wouter Christiaens General Manager and founder
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